BlackHeart Biohazards

Despite a ton of queer subtext, the actual canonical text of Ace Attorney is deeply homophobic and heteronormative.

Homosexuality and queerness are never directly referenced or mentioned at all in the series. There is never a moment in the series where it is directly discussed that non-straight is a thing you can be.

However, there are a few characters who are implied to be non-straight and these cases are handled in the same way every time.

• Male homosexual stereotypes: men who wear cosmetics, are flashy and limp wristed. These queer stereotype men are treated with derision by the characters and the text. At one point Phoenix Wright directly calls another man (Redd White) a “fruitcake” which is a slur against homosexual men.
• Implied lesbians: Specifically Lana Skye and Aura Blackquill. In both cases these characters are hinted to have romantic feelings for another woman. In both these cases, the characters around them do not reference this directly and instead act embarrassed on the woman’s behalf. Ema hurries to explain that Lana’s attraction to Mia is “an intellectual attraction”, and in court, everyone is nervous to explain to the judge that Aura was in romantic love with Metis and it’s never said directly, only hinted around with embarrassment.

In both cases, male and female, the characters of Ace Attorney act in a way that suggests that homosexuality in the Ace Attorney universe is something embarrassing and shameful.

At no point in the series is there ever a moment where homosexual behavior and presentation isn’t treated as something embarrassing and shameful.